home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9410o.zip
/
M94A2752.TXT
< prev
next >
Wrap
Text File
|
1994-10-25
|
2KB
|
35 lines
Document 2752
DOCN M94A2752
TI Targetting of HIV-DNA by probe DNA beads to improve PCR sensitivity.
DT 9412
AU Tano H; Fan K; Kitajima M; Kasai K; Hayashi T; Kondo M; Imai M; Japan
Synthetic Rubber Co., Ltd. Tsukuba Research Laboratory,; Tsukuba.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):232 (abstract no. PB0358). Unique
Identifier : AIDSLINE ICA10/94369823
AB OBJECTIVE: The HIV-DNA assay by PCR has been widely used. To increase
the sensitivity and specificity of conventional PCR method for detection
of HIV-DNA, latex beads bound with probe-DNA were applied to concentrate
and isolate the target DNA. METHODS: Probe-DNA against HIV-DNA was
covalently bound to latex bead with carboxylate groups on its surface.
This latex suspension was hybridized with a model specimen containing
several copies of HIV DNA in the volume of 30ml, and thereafter
concentrated to 30 microliters (1000 fold concentrated), by
centrifugation. Concentrated HIV DNA was used as the template for nested
PCR detection. RESULTS: Concentrated target DNA was successfully
detected by PCR, whereas the same specimen without concentration is
undetectable by PCR. The assay sensitivity was improved in the order of
100 to 1000. DISCUSSION AND CONCLUSIONS: In this study, it is
experimentally confirmed that probe-DNA bound latex bead could target
HIV-DNA to improve PCR assay sensitivity and reduce the nonspecific
bands. It can be applied for detection of HIV-DNA by PCR after pooling a
number of individual specimen into one with a larger scale, followed by
the concentration of the target DNA. In this way, a more efficient and
lower cost PCR diagnotics can be expected.
DE *DNA Probes DNA, Viral/*ANALYSIS HIV/GENETICS/*ISOLATION & PURIF
Latex Microspheres Polymerase Chain Reaction/INSTRUMENTATION/*METHODS
Sensitivity and Specificity MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).